Journal: STAR Protocols

Article Title: Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomics

doi: 10.1016/j.xpro.2026.104399

Figure Lengend Snippet:

Article Snippet: MillexTM-GP Filter Unit (0.22 μm Sterile) , Merck , Cat# SLGP033R.

Techniques: Virus, Plasmid Preparation, Sequencing, Recombinant, Modification, Saline, SDS-Gel, Protease Inhibitor, Staining, Sterility, Transduction, Software, Targeted Proteomics, Protein Purification, Mass Spectrometry

Journal: STAR Protocols

Article Title: Protocol for MR1-ligand cross-linking to discover metabolite antigens by proteomics

doi: 10.1016/j.xpro.2026.104399

Figure Lengend Snippet:

Article Snippet: MillexTM-HP Filter Unit (0.45 μm Sterile) , Merck , Cat# SLHP033R.

Techniques: Virus, Plasmid Preparation, Sequencing, Recombinant, Modification, Saline, SDS-Gel, Protease Inhibitor, Staining, Sterility, Transduction, Software, Targeted Proteomics, Protein Purification, Mass Spectrometry

Journal: STAR Protocols

Article Title: Protocol for antibody optimization and panel design in high-dimensional multiplexed immunofluorescence imaging

doi: 10.1016/j.xpro.2025.104236

Figure Lengend Snippet: Barcode-conjugated antibody validation by SDS-PAGE Representative gel electrophoresis showing unconjugated and barcode-conjugated antibody samples for CD8, GATA3, and PD-1. All antibodies resolve around 50 kDa, consistent with their expected molecular weight. Successful conjugation is indicated by an upward mobility shift of the barcode-conjugated antibodies compared to their unconjugated counterparts.

Article Snippet: 50 kDa MWCO centrifugal filter units , Fisher Scientific , Cat# UFC505096.

Techniques: Biomarker Discovery, SDS Page, Nucleic Acid Electrophoresis, Molecular Weight, Conjugation Assay, Mobility Shift